Generating multiplex gradients of biomolecules for controlling cellular adhesion in parallel microfluidic channels.

نویسندگان

  • Tohid Fatanat Didar
  • Maryam Tabrizian
چکیده

Here we present a microfluidic platform to generate multiplex gradients of biomolecules within parallel microfluidic channels, in which a range of multiplex concentration gradients with different profile shapes are simultaneously produced. Nonlinear polynomial gradients were also generated using this device. The gradient generation principle is based on implementing parrallel channels with each providing a different hydrodynamic resistance. The generated biomolecule gradients were then covalently functionalized onto the microchannel surfaces. Surface gradients along the channel width were a result of covalent attachments of biomolecules to the surface, which remained functional under high shear stresses (50 dyn/cm(2)). An IgG antibody conjugated to three different fluorescence dyes (FITC, Cy5 and Cy3) was used to demonstrate the resulting multiplex concentration gradients of biomolecules. The device enabled generation of gradients with up to three different biomolecules in each channel with varying concentration profiles. We were also able to produce 2-dimensional gradients in which biomolecules were distributed along the length and width of the channel. To demonstrate the applicability of the developed design, three different multiplex concentration gradients of REDV and KRSR peptides were patterned along the width of three parallel channels and adhesion of primary human umbilical vein endothelial cell (HUVEC) in each channel was subsequently investigated using a single chip.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A Novel Microfluidic Design to Generate Multiplex Gradients of Biomolecules by Visulized Biomolecule Patterning and Direct Cell Adhesion

Here we present a novel microfluidic design to generate multiplex gradients of biomolecules based on hydrodynamic resistances. Chemical gradients were generated within parallel microfluidic channels, in which a range of multiplex concentration gradients with different profile shapes were simultaneously produced. We could also successfully generate covalent surface gradients of biomolecules. IgG...

متن کامل

Covalently immobilized biomolecule gradient on hydrogel surface using a gradient generating microfluidic device for a quantitative mesenchymal stem cell study.

Precisely controlling the spatial distribution of biomolecules on biomaterial surface is important for directing cellular activities in the controlled cell microenvironment. This paper describes a polydimethylsiloxane (PDMS) gradient-generating microfluidic device to immobilize the gradient of cellular adhesive Arg-Gly-Asp (RGD) peptide on poly (ethylene glycol) (PEG) hydrogel. Hydrogels are fo...

متن کامل

A Microfluidic Programmable Array for Label-free Detection of Biomolecules

Title of Dissertation: A MICROFLUIDIC PROGRAMMABLE ARRAY FOR LABEL-FREE DETECTION OF BIOMOLECULES Peter Hume Dykstra, Doctor of Philosophy, 2011 Directed By: Professor Reza Ghodssi, Department of Electrical and Computer Engineering One of the most promising ways to improve clinical diagnostic tools is to use microfluidic Lab-on-a-chip devices. Such devices can provide a dense array of fluidic c...

متن کامل

Compact microfluidic structures for generating spatial and temporal gradients.

We present an improved microfluidic design for generating spatial and temporal gradients. The basic functional elements are bifurcated and trifurcated channels used to split flow between two and three channels, respectively. We use bifurcated channels on the exterior of the channel manifold and trifurcated channels in the interior with mixing tees to recombine flows. For N gradient-forming leve...

متن کامل

A gradient-generating microfluidic device for cell biology.

The fabrication and operation of a gradient-generating microfluidic device for studying cellular behavior is described. A microfluidic platform is an enabling experimental tool, because it can precisely manipulate fluid flows, enable high-throughput experiments, and generate stable soluble concentration gradients. Compared to conventional gradient generators, poly(dimethylsiloxane) (PDMS)-based...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Lab on a chip

دوره 12 21  شماره 

صفحات  -

تاریخ انتشار 2012